FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26073219
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC61250.012.01022411
TCAGGTA66350.011.4606097
TAGAACA71650.010.48746612
CAGGTAG71200.010.4586138
GGTAGAA73900.010.16753510
TATAGAA35350.09.3056961
AGGTAGA85100.08.9088979
GAATCGA42850.08.8306544
GGAAAAT111750.08.7503951
AGATCAG113400.08.5009064
AGAGATC132400.08.4730722
TAATCGA25800.08.468114
GTACTCG42500.08.3204194
CTCGAAT25600.08.0971711
AATCGAA50150.08.0392695
ACACTTT51650.07.8402218
CCCGAAT17900.07.8041231
ATCAGGT129950.07.7057196
AACAGTG101500.07.669292415
AATCGAG43200.07.61208445