FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25688981
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG24550.017.04558816
GAGTGTA30750.013.89979228
TCGTGTA31650.013.36391815
AGTGTAG31300.013.00857729
GTGTAGG33150.012.75924117
TGTAGGG33750.012.66573418
GTAGATC36100.011.96436332
TCTCGGT38150.011.43936437
GTGTAGA41450.010.58292330
ATCTCGG43450.010.56173136
GTAGGGA41200.010.32035919
TAGATCT42750.010.10324133
GTAGAAC45650.09.95446711
ATCGGAA45200.09.8664933
TAGGGAA42950.09.84772520
TCGGAAG51150.09.2471894
GAGCGTC57450.09.16303110
AGAGCGT56850.09.0225199
AATCGAA49950.08.7929615
TCAGGTA58400.08.7061787
Produced by FastQC (version 0.11.5)