FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29874908
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT34500.015.4752792
GCGTGAC46900.012.2027641
GTAGAAC48850.011.14440811
GTACTCG81100.010.359214
GTGACTC55100.010.1785963
GGAAAAT116950.010.1147791
AGTACTC86550.09.9411443
TCAGGTA59750.09.865027
TACTCGA85250.09.6699745
CTCGAAT24650.09.2321271
GCTCTAG66250.09.06633211
GGTAGAA63650.08.83589910
GAATCGA53300.08.7897154
TAGAACA62800.08.70535212
AAAATCC82900.08.504133
CAGGTAG67000.08.4951468
TGACTCT69900.08.4101164
GAATTGA71700.08.3560514
ACTCTGC79300.08.05880456
AATTGAC47350.07.9914935
Produced by FastQC (version 0.11.5)