FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28462532
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43650.011.33822911
CGTGACT28700.010.7533942
GTACTCG60950.010.7168464
TCAGGTA55250.09.935537
TACTCGA65650.09.8466815
AGTACTC73650.09.2970483
GGTAGAA55200.09.21036610
CAGGTAG57850.08.74996958
AATCGAA54500.08.596265
GGAAAAT110950.08.5902751
AGGTAGA61150.08.4248299
TAGAACA58750.08.34808212
GCGTGAC40750.08.2938791
GGTAGGC29500.08.1600967
AGAACGG194500.08.094723
GAATCGA45550.07.7139884
AAAATCC80650.07.6522723
CTCGAAT23150.07.59167431
GAATTGA63200.07.55547054
GTGACTC44900.07.52469973
Produced by FastQC (version 0.11.5)