FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891050
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT34350.014.9523382
GCGTGAC43750.012.6173631
GTAGAAC45050.010.8361411
GTACTCG71300.010.77214
GTGACTC51400.010.2538813
TCAGGTA54050.09.8230997
TACTCGA77650.09.7461565
AGTACTC80550.09.7028533
GGAAAAT111050.09.1909461
GAATCGA44150.09.0808154
GCTCTAG60150.08.9012511
TGACTCT62100.08.6321894
TAGAACA57600.08.59297112
AATCGAA50850.08.4601475
GGTAGAA60850.08.35528810
AAAATCC79600.08.31897453
CAGGTAG61500.08.3038288
CTCGAAT23150.08.2727231
ACTCTGC75700.08.1746426
GAATTGA67950.07.85587264
Produced by FastQC (version 0.11.5)