FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27079964
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC49400.012.75085411
CGTGACT19950.011.5179752
TCAGGTA58150.011.4518137
GTACTCG63250.010.8970744
TACTCGA66200.010.2753815
GGTAGAA63350.010.15615310
AGTACTC73200.010.0928213
TAGAACA63450.09.99899112
CAGGTAG66100.09.8020318
GGAAAAT132400.08.8834831
AGGTAGA74450.08.7327979
GCGTGAC31600.08.627781
AATCGAA50300.08.3738015
AATTGAC42450.08.2243785
AGATCAG110350.08.1646334
GAATTGA64950.08.1149474
AAAATCC92050.07.85472543
CATAAGC60200.07.84792857
GAATCGA40400.07.80540944
AACAGTG85400.07.718918315
Produced by FastQC (version 0.11.5)