FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n02_B3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n02_B3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24674084
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC42300.010.90241411
TCAGGTA54600.09.5593097
TAGAACA53050.09.07550912
GGAAAAT124300.08.9364661
GAATTGA66900.08.7535614
GTACTCG44350.08.4304744
AGGTAGA56750.08.40413959
CAGGTAG58550.08.2995498
GGTAGAA57250.08.29124410
AAAATCC84450.08.2413293
GGGAAAT61600.07.9738081
AAATCCT88950.07.87502534
AGTACTC53350.07.76822473
GAATCGA38050.07.75450664
CTCGAAT20500.07.69368031
GGAGAAT82300.07.5561271
AATCCGC29900.07.53297145
AATCGAA49400.07.52306035
AGATCAG99400.07.3870114
CGTGACT20450.07.38056372
Produced by FastQC (version 0.11.5)