FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567117
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9450.032.57684315
CGTCTGA10050.030.6318716
CACACGT10800.028.71247712
CACGTCT12000.025.84122814
ACACGTC11900.025.68072513
CAGTCAC8700.022.54822527
TCGGAAG17950.021.788013
AGCACAC14200.021.68929110
ATCGGAA18300.021.4941222
CCAGTCA10350.020.91429726
CCGAGAC6150.018.6935129
GATCGGA21350.018.5312391
CGGAAGA21350.018.4235324
GCACACG16600.018.41806211
GAGACCG7050.016.91390231
ACCGATC6450.016.3865934
CTCCAGT16150.016.05633424
AACTCCA20050.015.28691622
AGCGGTT8950.014.81979211
CGAGACC8750.013.65045330
Produced by FastQC (version 0.11.5)