FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19490989
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG30500.024.7850715
AGTCACG31000.023.9493728
CGTCTGA31700.023.56342516
CTCGTAT31700.021.93563542
TCGTATG33650.020.86585243
TCGGAAG43550.018.0854683
CGTATGC39650.017.8218644
ATCGGAA44600.017.7606032
GTCACGA42550.017.34276229
CACGTCT47100.016.38381414
CAGTCAC49200.015.5934927
CACACGT49800.015.58588712
TCTCGTA53100.013.60349141
TCACGAT54700.013.57287130
ACACGTC58250.013.51804513
CCAGTCA62750.012.22629126
CGGAAGA68200.011.9446684
GATCGGA66900.011.9430611
ACGATCA65350.011.77404332
AGCACAC70950.011.3837110
Produced by FastQC (version 0.11.5)