FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20757583
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43600.027.50349615
CGTCTGA45750.026.06395516
CTCGTAT33050.022.0607442
TCGGAAG56900.021.4357593
TCGTATG34800.020.95207243
GTCACAC55650.020.69917329
ATCGGAA58300.020.6894072
CACACGT60050.020.08148412
CACGTCT61350.019.9126614
CAGTCAC62150.018.82420727
AGTCACA65050.017.88085728
ACACGTC72650.016.90834213
CGTATGC42700.016.54874644
CACACTT70500.016.49877531
AATCTCG56250.016.0410939
CCAGTCA73550.015.6924326
CGGAAGA83000.015.21025854
TCCAGTC78600.015.05631725
GATCGGA81800.015.0225071
TCACACT78600.014.71261430
Produced by FastQC (version 0.11.5)