FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26073219
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC361420.13861732991235184TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG68600.031.45201115
CGTCTGA74300.028.9489116
CTCGTAT65600.027.4430642
TCGTATG67550.026.41846543
ATCGGAA83750.025.9562052
TCGGAAG84500.025.8589823
CGTATGC70550.024.68905644
CACACGT91400.024.02412412
CACGTCT92800.023.5162314
ACACGTC98850.022.55490113
CAGTCAC103000.020.7510627
GATCGGA106900.020.5695931
TCTCGTA90450.020.17651641
AGTCACC107050.020.1337428
CGGAAGA117350.018.9462284
TCCAGTC115450.018.66919125
CCAGTCA114950.018.65253426
GTATGCC98400.018.31908445
GTCTGAA123900.017.75949717
ACTCCAG133200.016.8234123
Produced by FastQC (version 0.11.5)