FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25688981
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1973840.7683605667348191TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG271550.039.23057615
CGTCTGA274700.038.69122716
AGTCACG268150.038.6613828
ATCGGAA283500.037.9266052
CTCGTAT254900.037.78524842
TCGGAAG284350.037.765753
GTCACGC277800.037.36712629
TCGTATG258000.037.0010943
TATCTCG273750.036.6598139
CACACGT292500.036.52741612
CACGTCT294000.036.3793214
ACACGTC297350.036.03755613
CGCCAAT284050.036.03031533
CGTATGC262400.035.9775644
ACGCCAA284100.035.9764632
CACGCCA295400.034.99628431
GTATGCC271100.034.9312445
CAGTCAC301400.034.66604627
TCACGCC301600.034.56019630
GATCGGA313550.034.504231
Produced by FastQC (version 0.11.5)