FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29874908
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45350.022.8707315
TCGTATG45350.022.47929643
CGTCTGA47750.021.53302416
CTCGTAT46450.021.0742242
CGTATGC59000.017.20232244
TCGGAAG65700.016.2697683
GTCACAC64450.016.0925829
ATCGGAA66450.016.0522692
CACGTCT70800.015.06222514
CACACGT75650.014.780580512
CAGTCAC72000.014.03028927
AGTCACA77300.013.24270528
AGTGATC76300.013.15229736
CACAGTG81500.012.56034233
TCTCGTA82300.012.4681341
ACACGTC90200.012.24669713
CCAGTCA88450.011.67528826
ACACAGT88050.011.65153632
GTATGCC94150.011.04303745
CGGAAGA103700.010.9371674
Produced by FastQC (version 0.11.5)