FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28462532
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48750.021.87548415
CGTCTGA52550.020.1654316
CTCGTAT42900.019.82801642
TCGTATG44500.019.62144343
AGTCACT59800.017.68247228
TCGGAAG68900.015.8409153
ACTGACC67650.015.69722332
ATCGGAA70550.015.4385352
CACGTCT72000.015.06124514
CGTATGC57800.014.87289644
CAGTCAC73450.014.67230827
AATCTCG69350.013.8217239
CACACGT81750.013.56767712
GTCACTG84050.012.84842529
ACACGTC87500.012.72751113
CACTGAC87350.012.44035731
TCTCGTA72500.012.35312641
CCAGTCA86750.012.26724726
TCCAGTC94750.011.51643425
CGGAAGA102000.011.4725754
Produced by FastQC (version 0.11.5)