FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891050
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG50350.027.48033732
ACGTCTG51400.027.4445615
CGTCTGA56050.025.24849116
CTCGTAT50750.025.1418842
TCGTATG50650.024.74815443
TCACTTA60600.022.79513430
CTTAGGC61550.022.55295833
TTAGGCA61300.022.46255534
AGTCACT62800.022.1397628
GTCACTT64800.021.38707229
ATCGGAA70300.020.7110442
TCGGAAG70700.020.6575283
CACGTCT73300.019.85840614
CGTATGC64650.019.52816444
CACACGT75550.019.08831812
CAGTCAC77250.018.17347327
ACACGTC90550.016.07532913
CCAGTCA89450.015.87087526
TCTCGTA83850.015.646141
TAGGCAT93050.015.039803535
Produced by FastQC (version 0.11.5)