FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27079964
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44600.024.76828215
CTCGTAT42000.023.57532742
CGTCTGA47100.023.31104716
TCGTATG43550.022.58198243
CGTATGC48800.019.82975644
TCGGAAG57000.019.4636363
ACCGATG56600.018.72192632
ATCGGAA60200.018.3542612
CACCGAT60400.017.54405831
GATGTAT62400.017.19874235
TATCTCG61450.016.80769739
CACACGT69250.016.17887912
CACGTCT70350.016.14976114
CAGTCAC71900.015.48899327
GTATCTC76050.014.29093238
TCTCGTA71300.014.17100741
ACACGTC82050.014.03880813
AGTCACC82300.013.96887828
TGTATCT84750.013.16905337
CCAGTCA84800.013.15929626
Produced by FastQC (version 0.11.5)