FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l06_n01_B3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l06_n01_B3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24674084
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43950.025.18614215
CGTCTGA45450.024.15710316
TCGTATG45400.021.90929443
CTCGTAT44000.021.73631142
ATCGGAA55800.020.0032922
CGTATGC50450.019.49319644
TCGGAAG58700.019.1300663
CACACGT63800.017.7021912
CACGTCT65700.017.29298614
CAGTCAC72450.015.58893127
TCACGAT70600.015.48804735
GTCACAT73700.015.01908229
ACACGTC75650.014.98875613
TCTCGTA66250.014.91143441
AGTCACA75300.014.58040828
GATCGGA79000.014.1878931
ATCACGA77850.014.01680734
CCAGTCA86850.012.7711226
GTATGCC82500.012.629683545
GTCTGAA89400.012.53287617
Produced by FastQC (version 0.11.5)