FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17935491
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA5300.022.075528
GTAGATC5650.021.877932
CACTCTT10050.021.5437932
AGTGTAG5550.021.08104929
TCTCGGT6600.019.05454837
GTGTAGA6350.018.77923630
TAGATCT6850.018.34531833
TGTAGGG7700.016.65876218
GTGTAGG7950.015.86535917
GTAGGGA8050.015.666471519
CTCGGTG10000.015.49638738
TGTAGAT8000.015.46291631
TTCCCTA5750.014.8790978
TCGTGTA8450.014.66460415
GTAGAAC3550.014.59573711
TAGGGAA8450.014.39227720
ATCGGAA13400.013.8014912
TCCCTAC5900.013.7317589
AGCGGTT5800.013.5905610
TCAGGTA4300.013.0916917
Produced by FastQC (version 0.11.5)