FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815446
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21950.014.1669522
GTAGAAC29050.012.62303311
GCGTGAC26050.012.5449851
TCAGGTA36550.011.3874177
GTACTCG53650.09.8668014
TAGAACA37650.09.8599612
AGTACTC54850.09.856753
GTGACTC34950.09.5392373
TACTCGA55850.09.3973275
GGTAGAA40050.09.38024710
GAATTGA44600.09.2931134
CAGGTAG41850.09.192188
TGACTCT39300.08.6549114
AATCGAA34700.08.5038095
AATTGAC28800.08.4470085
GCTCTAG39200.08.43632911
AAAATCC62600.08.3485823
GGAAAAT90150.08.3000781
TCACACG19900.08.25326915
CTCTATA40150.08.17995945
Produced by FastQC (version 0.11.5)