FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25223602
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43450.011.18365911
CGTGACT25650.010.98137952
TCAGGTA50100.010.508017
GAATCGA39450.09.5928064
GGAAAAT124000.09.5060781
GTACTCG54950.09.4285194
AAAATCC86900.09.2285123
GCGTGAC33150.09.1784971
TAGAACA53400.09.1005712
TACTCGA60000.08.6723245
AGTACTC63650.08.6002163
CAGGTAG57650.08.5460368
AATCGAA44300.08.5424075
GGTAGAA58450.08.4286510
AATCCTT81400.08.1910965
AGTGTGA66300.08.17823514
AAATCCT97450.08.1597394
GAATTGA59750.08.0678764
GGGAAAT56600.08.0437091
GTGTGAG65550.07.997225315
Produced by FastQC (version 0.11.5)