FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26063871
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29800.012.9307042
GTAGAAC50100.011.04640411
GCGTGAC41650.010.0650571
TCAGGTA59350.09.8937287
GTGACTC43850.09.6579233
GGAAAAT107300.09.3051271
GGTAGAA62850.09.19864610
GTACTCG52950.09.1038824
CAGGTAG65300.08.7507098
TACTCGA55700.08.5734115
TAGAACA64050.08.53586112
GCTCTAG50850.08.27321211
AAAATCC76500.08.215583
TGACTCT56050.07.9573494
GAATTGA62950.07.94394164
GGTAGGC26250.07.8849467
CTATAAG29450.07.80177974
AGTACTC65700.07.71458633
AGATCAG103000.07.6543734
CTCGAAT23350.07.6253271
Produced by FastQC (version 0.11.5)