FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21804300
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG16650.015.94484916
CGTGACT24500.013.5203122
AGTGTAG19350.012.32278329
GTAGAAC42600.012.30461811
GAGTGTA19650.011.79121628
TCGTGTA23050.011.61525315
TCAGGTA46850.011.5251967
TGTAGGG23750.011.36737918
GTGTAGG24400.011.34143417
GCGTGAC32550.010.5938981
GGTAGAA50600.010.40315810
TAGAACA51350.010.16483212
GTACTCG50200.010.0513924
GTAGATC28250.010.0331232
CAGGTAG53500.09.8817968
TAGATCT28400.09.82171333
TACTCGA50950.09.7264515
GTGACTC38200.09.5532143
GTGTAGA27900.09.51396930
GAATCGA32850.09.3943574
Produced by FastQC (version 0.11.5)