FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25793070
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43150.013.24265511
CGTGACT25400.012.7751342
TCAGGTA52000.011.5519067
GCGTGAC35850.010.5616121
GTACTCG59150.010.2443054
TAGAACA56350.010.101412
GGTAGAA57400.09.91531210
TACTCGA62950.09.5542245
CAGGTAG60550.09.5115518
AGTACTC66300.09.4796913
CTCGAAT19650.09.4050561
GGAAAAT105100.09.3710621
GAATTGA57200.09.21524
AATCGAA44050.08.8978125
GAATCGA40450.08.6874254
AAAATCC80600.08.3288433
GTGACTC43150.08.248593
AGATCAG92900.08.2199454
GGGAAAT52350.08.1368291
CTATAAG25250.07.85066134
Produced by FastQC (version 0.11.5)