FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25327756
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28150.012.7283292
GCGTGAC35100.010.6593341
GTACTCG56000.09.8553854
GTAGAAC37000.09.8498911
TCAGGTA45500.09.5431257
GGAAAAT108750.09.5128911
AGTACTC62400.09.0255063
AAAATCC74400.08.8415023
TACTCGA60900.08.655475
GTGACTC45300.08.354633
GAATTGA59200.08.2191624
CAGGTAG48050.08.193478
GCTCTAG46950.08.0978411
GGGAAAT51000.07.68967871
TAGAACA50900.07.5584912
GGTAGAA49850.07.536137610
AATTGAC41050.07.40825
GAAAATC86350.07.17667062
AGAACGG161350.07.1485363
GAATCGA42250.07.14452934
Produced by FastQC (version 0.11.5)