FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26935912
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32050.014.6245632
GCGTGAC39700.012.3196971
GTACTCG65850.010.3310714
GGAAAAT126450.010.0172441
GTAGAAC44450.09.91991911
GTGACTC49200.09.7527173
TACTCGA69500.09.7235175
AAAATCC88300.09.7202053
AGTACTC73950.09.5044623
AATCGAA49050.09.2308985
TCAGGTA54150.08.9326927
AAATCCT96450.08.8751474
GAATCGA44200.08.86790854
GCTCTAG57150.08.8570711
TGACTCT56750.08.7724444
GAAAATC107750.08.4282112
CTCGAAT22800.08.4026231
GGGAAAT60350.08.2162981
AATCCTT81100.08.1938515
TAGAACA56500.07.924325512
Produced by FastQC (version 0.11.5)