FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30686496
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT32150.013.247512
GCGTGAC42200.011.6433591
GTAGAAC45800.09.77484911
GTACTCG72950.09.2945744
TCAGGTA53750.09.2084647
GGAAAAT148500.09.0307681
GAATCGA43950.08.9694834
GTGACTC51350.08.9057123
GAATTGA74500.08.6476494
AGTACTC81900.08.4993883
AATTGAC48400.08.4705195
TACTCGA77200.08.4326595
AAAATCC103200.08.4041573
AATCGAA52400.08.25385
TCACACG31450.08.01222415
CTCGAAT23750.07.97168061
GGGAAAT64100.07.946661
GCTCTAG62000.07.91019611
ATTGACC50200.07.8897216
TAGAACA58200.07.80879612
Produced by FastQC (version 0.11.5)