FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n02_E3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n02_E3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30057544
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT35600.014.8117352
GCGTGAC44550.012.5464271
GTAGAAC40750.011.70387211
GTGACTC53550.010.4325493
TCAGGTA51850.09.762957
AATTGAC54650.09.5213155
GCTCTAG62650.09.51581711
GAATTGA77500.09.4753994
GTACTCG81250.09.4539354
AGTACTC87050.09.1866643
TGACTCT64550.08.96847254
GGAAAAT134850.08.8079671
ATTGACC52700.08.7111176
TACTCGA84400.08.7006215
AAAATCC98450.08.6034053
GAATCGA44550.08.4946044
TAGAACA57150.08.42466812
GGTAGAA59700.08.32752810
TCACACG32900.08.27455715
AATCGAA53850.08.1150485
Produced by FastQC (version 0.11.5)