FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17935491
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9800.033.69903615
CGTCTGA10200.032.5985816
CACGTCT12100.027.66477214
CACACGT12850.026.39976312
ACACGTC13150.025.28495413
CAGTCAC9200.023.52833727
AGCACAC15250.022.11017810
TCGGAAG19600.021.2079523
ATCGGAA19850.021.1672382
CCAGTCA11750.020.7253126
GATCGGA22250.019.2911951
GCACACG17850.019.1415111
CGGAAGA23600.017.8037994
ACCGATC6600.017.71566634
CCGAGAC7050.017.58930229
CTCCAGT16700.017.28272624
AACTCCA19750.015.74694422
GAGACCG7650.015.58903331
AGCGGTT9050.014.40622511
ATGCCGT10550.012.45543645
Produced by FastQC (version 0.11.5)