FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19815446
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG32300.027.79166828
ACGTCTG32850.027.7370715
CGTCTGA34100.026.2588816
CTCGTAT32400.025.49190342
TCGTATG35650.023.42129943
CGTATGC40700.020.57051344
ATCGGAA46000.020.20152
TCGGAAG46950.019.7927363
GTCACGA45650.019.71358729
CACGTCT48250.019.16382214
CACACGT52150.017.6875312
CAGTCAC53500.017.11548627
TCTCGTA52250.016.53960241
ACACGTC61200.015.32929913
CCAGTCA62150.014.8059526
TCACGAT65850.013.97399230
ACGATCA66950.013.81199232
AGCACAC71050.013.55243610
GAGCACA72400.013.5172519
TCCAGTC68450.013.50897725
Produced by FastQC (version 0.11.5)