FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25223602
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG44000.026.02555315
CGTCTGA48450.023.63597316
TCGTATG37150.019.74963243
CTCGTAT38300.019.7435942
ATCGGAA59800.019.6408442
TCGGAAG61850.019.0626133
CACGTCT62750.018.82256114
GTCACAC61300.018.27749429
CACACGT69350.016.9987912
CAGTCAC69850.016.20115327
CGTATGC42850.016.12468344
AGTCACA74500.015.12939328
ACACGTC82750.014.51798613
AATCTCG63000.014.46671439
CACACTT79700.014.11492131
CCAGTCA83450.013.66882326
GATCGGA88450.013.5609721
ACACTTG86800.013.11588432
TCTCGTA63650.012.69350641
TCCAGTC92200.012.66446425
Produced by FastQC (version 0.11.5)