FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26063871
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC330450.12678469748411508TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG69450.030.06202115
CGTCTGA72600.028.75800916
CTCGTAT63050.028.30423242
TCGTATG63050.027.41291643
ATCGGAA86300.024.639022
TCGGAAG88300.024.0554643
CACGTCT89100.023.8613914
CACACGT91800.023.2821212
CGTATGC73900.022.90107244
CAGTCAC95950.021.6893627
ACACGTC104100.020.89860713
AGTCACC107950.019.48661628
GATCGGA110250.019.3710981
TCTCGTA94650.019.16362641
CCAGTCA110800.018.86383826
TCCAGTC117300.017.95282625
CGGAAGA122050.017.86444
AGCACAC125900.017.15485810
GTCTGAA125200.017.071517
GTATGCC103350.017.05046545
Produced by FastQC (version 0.11.5)