FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21804300
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1364280.6256930972331146TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG187350.039.14748415
CGTCTGA189350.038.7114716
AGTCACG187400.038.17679628
CTCGTAT178700.037.29007742
TCGGAAG200650.036.9497073
ATCGGAA201100.036.822272
TCGTATG178800.036.79176743
GTCACGC195300.036.71366529
CACGTCT204200.036.0162214
TATCTCG193900.035.78114339
CACACGT209600.035.0883212
CGTATGC185950.034.94137244
CAGTCAC207800.034.5378327
CGCCAAT203100.034.51869633
ACACGTC215800.034.25745413
ACGCCAA204750.034.1525432
CACGCCA208900.034.02334631
CCAGTCA213300.033.6368726
TCTCGTA202450.033.10429441
GATCGGA225600.033.078051
Produced by FastQC (version 0.11.5)