FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25793070
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1123270.43549294442266856TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG144800.038.92061215
CGTCTGA146300.038.39950616
TCGTATG145950.036.34502843
CTCGTAT145200.036.0818742
ATCGGAA163700.034.884752
TCGGAAG164750.034.758023
CGTATGC154700.034.39141544
CACGTCT166050.034.0616114
GTCACAC159650.033.9902829
CACACGT169800.033.38886312
CAGTCAC170250.031.96668827
AGTCACA169950.031.89023828
ACACGTC179200.031.73787513
AGTGATC172300.030.3368836
TCTCGTA173800.030.27383441
CACAGTG177000.030.26536833
CCAGTCA180950.030.12662326
GTATGCC178850.029.9745945
ACACAGT179550.029.8981332
GATCGGA193850.029.5563031
Produced by FastQC (version 0.11.5)