FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25327756
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG40750.019.37844815
CGTCTGA42850.018.4292916
CTCGTAT33550.017.64157342
TCGTATG37000.015.63204443
AGTCACT49800.015.540627528
TCGGAAG55650.014.39409353
CACGTCT56600.014.34925614
ATCGGAA58300.013.8170062
ACTGACC54900.013.76986132
CAGTCAC60300.013.24513927
CACACGT64850.012.66255912
CGTATGC46450.012.20956144
AATCTCG58350.012.10994839
ACACGTC73250.011.33332413
GTCACTG71100.011.16991129
CACTGAC71650.010.92762231
CCAGTCA77050.010.07396526
GACCAAT78400.09.81469735
TCTCGTA64750.09.80127441
TATAGGC49850.09.74841627
Produced by FastQC (version 0.11.5)