FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26935912
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG39600.024.43106332
ACGTCTG41550.023.98668915
CGTCTGA43800.022.34420416
CTCGTAT40500.021.44895642
TCGTATG42150.020.61019943
TCACTTA53000.018.42323930
CTTAGGC53200.018.31243733
TTAGGCA52600.018.26467734
TCGGAAG55850.018.2096043
AGTCACT55800.017.65969528
GTCACTT55950.017.17022329
CACGTCT61100.016.9008414
ATCGGAA59600.016.8373532
CGTATGC52500.016.54716744
CACACGT67600.014.97623512
CAGTCAC68950.014.2266827
ACACGTC77800.013.44652813
TCTCGTA67700.013.26345141
TAGGCAT79800.012.37750535
CACTTAG79700.012.13889731
Produced by FastQC (version 0.11.5)