FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30686496
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41050.021.4839815
CGTCTGA45700.019.05210516
CTCGTAT42500.018.37470442
TCGTATG42250.018.11115843
ATCGGAA58450.015.4752582
TCGGAAG61000.014.9390013
CGTATGC51750.014.74298844
GATGTAT57800.014.67509235
CACGTCT63400.014.51355414
ACCGATG59850.014.39782832
TATCTCG59150.014.34312539
CACCGAT61100.014.06645131
CACACGT70100.012.99800612
CAGTCAC71400.012.35198627
GTATCTC73600.012.16750838
ACACGTC85150.011.22907513
AGTCACC83950.010.80007828
TGTATCT86400.010.57349537
TCTCGTA78600.010.2790241
CCAGTCA91250.09.73906826
Produced by FastQC (version 0.11.5)