FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l05_n01_E3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l05_n01_E3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30057544
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51250.027.78754615
CGTCTGA54800.026.02927216
TCGTATG55800.022.90854643
CTCGTAT54300.022.38033142
CGTATGC60100.021.19474844
ATCGGAA71600.020.1752742
TCGGAAG71950.020.1084043
CACGTCT72100.020.06389814
CACACGT74800.019.61036312
CAGTCAC76200.018.6600427
GTCACAT81550.017.10469429
AGTCACA85550.016.4889228
ACACGTC97250.015.26841513
CCAGTCA95600.014.80292626
TCTCGTA86750.014.73499441
ATCACGA97300.014.42923134
GATCGGA104400.014.1625371
TCACGAT98600.014.0792335
AGCACAC106850.014.02294910
CGGAAGA111150.013.5429744
Produced by FastQC (version 0.11.5)