FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552748
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT9950.026.055412
TCTCGGT5150.023.10994337
GAGTGTA5450.021.05255528
TAGATCT5800.019.73357633
AGTGTAG5700.019.73354529
TTCCCTA6100.019.195288
GTAGATC6000.019.10570132
GTGTAGA5800.019.00544530
TCCCTAC5850.017.6997159
TGTAGGG7700.016.37306418
TCGTGTA7600.016.31112115
GTGTAGG7700.016.0974117
CCGTTAC1004.116679E-415.77730416
TGTAGAT7550.015.194077531
GTAGGGA7900.014.82066819
TCGGAAG13650.014.6960013
TTTCCCT10850.014.5283137
ATCGGAA14000.014.4923112
AGCGGTT7450.013.91092110
CTCGGTG9400.013.85621238
Produced by FastQC (version 0.11.5)