FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19989053
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22050.015.6311462
GCGTGAC29700.012.2941771
GTAGAAC28000.011.24938611
GTGACTC34350.010.75326253
CTAGCAC38650.09.54748514
GCTCTAG39700.09.29420411
TCAGGTA36950.09.1957377
TGACTCT42700.09.0191654
GGAAAAT86750.08.9897481
GGTAGAA36650.08.96303510
GTACTCG49700.08.745794
TACTCGA49450.08.7443735
TAGAACA37300.08.50532312
AATCGAA38550.08.4710535
AGTACTC56800.08.3667583
GAATCGA30800.08.2627734
GAATTGA51550.08.1697924
CAGGTAG41950.07.99230058
AAAATCC61450.07.9902013
GGGAAAT42950.07.97666451
Produced by FastQC (version 0.11.5)