FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24983049
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25600.012.9354812
GCGTGAC34800.010.945721
GTAGAAC39500.010.13872811
GTACTCG55000.010.1142014
TACTCGA55400.09.83785255
GTGACTC36500.09.3793983
GAATCGA39100.09.2735594
GGAAAAT107100.09.1545421
AAAATCC76550.09.1209653
AGTACTC63050.09.1091813
TCAGGTA50050.08.9020037
AATTGAC43000.08.3800425
CTAGCAC42850.08.29657814
GGTAGAA50600.08.27064810
GCTCTAG47600.08.22437511
GAATTGA64250.08.0271184
TAGAACA51650.08.01543612
CTCGAAT22000.07.9911411
AATCGAA44000.07.9336625
CAGGTAG54150.07.89544448
Produced by FastQC (version 0.11.5)