FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184095
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47400.011.29692311
TCAGGTA53700.010.5177147
GTACTCG44000.09.57164
GGTAGAA58500.09.53841810
CAGGTAG59450.09.386768
TAGAACA59800.09.10539912
TACTCGA47000.08.6730715
GAATTGA66000.08.1896054
CGTGACT21600.08.1348682
CTATAAG29150.08.1123444
GGAAAAT101700.08.0891781
AGATCAG100200.08.0240964
AATTGAC41450.07.98702345
AGAGATC112300.07.96380042
AGTACTC59500.07.64642673
TATAAGG33100.07.62047775
GGACTTA36900.07.5071384
AGAACGG154900.07.44462253
AGGTAGA75000.07.35044779
AACGGCC113350.07.3117025
Produced by FastQC (version 0.11.5)