FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15729322
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG17850.018.151716
TGTAGGG19850.016.54941218
GAGTGTA19900.015.93813528
GTAGATC21800.015.27040132
TCGTGTA21250.015.24742615
GTGTAGG22350.014.49699417
TAGATCT23350.014.25623433
AGTGTAG21700.013.99344929
GTAGGGA26400.012.35801719
TCTCGGT25600.012.29923437
ATCGGAA28150.012.16171553
TAGGGAA26750.012.02951320
GTGTAGA26950.011.93515330
TCGGAAG30500.011.5932334
GTAGAAC26050.011.31422311
ATCTCGG31950.010.91065536
GTCGTGT32650.010.40603614
TCAGGTA32100.010.0943127
GAGCGTC40700.09.89547110
GGTAGAA31550.09.770110
Produced by FastQC (version 0.11.5)