FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25661862
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27450.015.7563812
GCGTGAC37800.012.580591
GTAGAAC35500.012.10511611
GTACTCG60150.010.4088044
TCAGGTA45250.010.3932597
GTGACTC44800.010.356343
TACTCGA60500.09.90173155
GGAAAAT106850.09.4285161
AGTACTC67750.09.3414463
AATTGAC36850.09.2894715
CTAGCAC48150.09.20594914
TAGAACA47450.09.19921212
TATAAGG31100.09.1966195
CTCGAAT23800.09.185551
GCTCTAG52700.09.05084611
TGACTCT55650.08.9842014
CTATAAG30600.08.9054454
GAATCGA40000.08.8958774
AAAATCC77500.08.8927643
GGTAGAA49350.08.84493410
Produced by FastQC (version 0.11.5)