FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22204932
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT21050.014.3401392
GTACTCG55950.010.9085494
TACTCGA57600.010.5177245
GTAGAAC37050.010.50555911
GCGTGAC30750.010.4812951
AGTACTC61150.010.2024993
TCAGGTA42300.09.9477117
GGAAAAT97700.09.6659271
AATCGAA41850.09.0407775
GTGACTC35750.08.9461373
TAGAACA44650.08.86900612
TAAATCG8850.08.6528443
AAAATCC75050.08.5529793
CAGGTAG49050.08.3951448
GGTAGAA48700.08.26993310
AGTTACG6857.2759576E-128.2139426
CTAGCAC38200.08.18741814
CTCGAAT21750.08.1863791
TGACTCT43800.08.0727844
AAATCCT81200.07.9324594
Produced by FastQC (version 0.11.5)