FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30444076
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28700.013.8928052
GCGTGAC40250.010.8634041
GTAGAAC42900.09.85969811
GGAAAAT123000.09.0521541
GTACTCG75150.08.9605874
AGTACTC84250.08.5546353
GGGAAAT65300.08.5253821
TACTCGA77950.08.4941895
TCAGGTA53900.08.4746637
GTGACTC51700.08.3643473
GCTCTAG55000.08.2223611
AAAATCC90600.08.0793523
GAATCGA49250.08.0482374
AATTGAC44800.08.0432715
CTAGCAC52350.07.951494714
GGACTTA41750.07.87572674
GAATTGA75900.07.7444934
ATTGACC44700.07.70337776
GGGGAAT59450.07.65825131
GGTAGAA55800.07.499935610
Produced by FastQC (version 0.11.5)