FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27298786
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27400.015.7037772
GCGTGAC38950.011.3418811
GTAGAAC38200.010.954988511
GTACTCG70900.010.7051474
TACTCGA71350.010.5112915
GTGACTC46250.010.3730463
AGTACTC79000.010.0928693
GCTCTAG52900.09.01659411
TCAGGTA48700.09.0101757
GGAAAAT114700.08.9606171
TGACTCT55200.08.93544
TATAAGG31450.08.736655
CTAGCAC51100.08.63043214
TAGAACA49300.08.58024112
GGTAGAA52200.08.57759610
AAAATCC79150.08.3094143
CTATAAG29700.08.2656884
CTCTATA58950.08.12619245
ACTCGTG58650.08.0142436
CACTCGT59300.08.00240735
Produced by FastQC (version 0.11.5)