FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n02_D3S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n02_D3S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24965362
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25400.013.7468672
GCGTGAC31250.011.6119621
GTAGAAC38450.010.82518311
GTACTCG67650.010.220394
GTGACTC38200.010.141153
TACTCGA66950.010.0916545
GGAAAAT116500.09.8667471
GAATCGA38150.09.7406144
AGTACTC73850.09.5763793
TCAGGTA46400.09.5536637
CTCGAAT21000.09.5521351
AAAATCC86500.09.2434573
CTAGCAC42500.09.21202214
GCTCTAG44400.08.91845411
AATCGAA47450.08.5433455
AAATCCT89550.08.5257194
TAGAACA49350.08.34344812
GGTAGAA51850.08.24480410
TGACTCT48850.08.2063934
GAATTGA62850.07.95508054
Produced by FastQC (version 0.11.5)