FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18552748
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9300.034.55026615
CGTCTGA9500.033.58618516
CACACGT12150.026.4463112
CACGTCT12500.026.24509614
ACACGTC13050.024.79461113
AGCACAC14150.023.3560410
CAGTCAC9800.021.84891527
ATCGGAA19550.020.8067322
CCAGTCA11350.020.65320426
TCGGAAG19600.020.409673
ACCGATC5700.020.11660634
GCACACG17100.019.32678211
CCGAGAC6450.018.8708329
GATCGGA22150.018.672061
CTCCAGT15000.018.6332524
CGGAAGA21850.018.3079874
GAGACCG7200.017.49561731
AGACCGA7350.016.83192832
AACTCCA19700.015.89881322
AGCGGTT8500.015.60527311
Produced by FastQC (version 0.11.5)