FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19989053
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG34100.025.66480428
ACGTCTG35750.025.04471615
CGTCTGA37050.024.22667116
CTCGTAT35400.023.52568242
TCGTATG37750.022.47955543
CGTATGC42250.020.1385144
TCGGAAG46750.020.1172643
ATCGGAA46700.019.849732
GTCACGA45700.019.59427529
CACGTCT50050.018.60810314
CACACGT53500.017.07175312
CAGTCAC53450.017.04661427
TCACGAT57550.015.71603730
TCTCGTA55050.015.250854541
ACACGTC63250.014.9736413
ACGATCA67300.013.64012232
CCAGTCA66400.013.55328226
GATCGGA72450.013.2937351
CGGAAGA73750.013.2404254
GTATGCC68650.012.91883545
Produced by FastQC (version 0.11.5)