FastQCFastQC Report
Mon 13 May 2019
CCY3TACXX_l04_n01_D3S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3TACXX_l04_n01_D3S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24983049
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG43100.025.94148315
CGTCTGA47950.023.27065816
TCGGAAG56450.020.0884973
TCGTATG38200.020.03452143
ATCGGAA57250.019.8471262
CTCGTAT39700.018.93667642
CACGTCT61500.018.36284314
CACACGT62400.018.20611212
GTCACAC60800.018.09498829
CGTATGC42450.017.33934244
CAGTCAC71750.015.42681227
ACACGTC78700.014.74977513
AGTCACA75100.014.70906428
AATCTCG63700.014.48481939
CACACTT80850.013.77501631
CCAGTCA86150.012.8487326
CGGAAGA96250.012.6934584
TCACACT89050.012.68304930
GATCGGA92000.012.6455931
TCTCGTA65000.012.43165341
Produced by FastQC (version 0.11.5)